Author + information
- Received July 1, 1994
- Revision received January 12, 1995
- Accepted April 7, 1995
- Published online September 1, 1995.
- Kunihisa Miwa, MD*,
- Yuko Miyagi, MD and
- Masatoshi Fujita, MD
- ↵*Address for correspondence: Dr. Kunihisa Miwa, The Second Department of Internal Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-01, Japan.
Objectives The purpose of this study was to investigate the peroxidative susceptibility of plasma low density lipoprotein in patients with active variant angina and to compare it with that in subjects without coronary spasm.
Background Oxidized or modified low density lipoprotein can impair endothelium-dependent vasoregulation and has atherogenic properties; it may be related to the genesis of coronary artery spasm by potentiating agonist-induced vasoconstriction.
Methods The sensitivity of plasma low density lipoprotein for cupric ion (Cu2+)—induced peroxidation was examined. Low density lipoprotein was isolated from plasma in 112 patients: 21 with active variant angina, 18 with inactive variant angina without anginal attacks during the preceding 6 months, 39 with significant organic coronary artery stenoses but without rest angina and 34 control subjects without coronary artery disease. Lipid peroxidation products in low density lipoprotein were assayed as thiobarbituric acid-reactive substances before and after incubation with various concentrations of Cu2+at 37°C for 24 h.
Results Significantly higher levels of generation of thiobarbituric acid—reactive substances from plasma low density lipoprotein after incubation with Cu2+were seen in patients with active variant angina than in patients in the other three groups. The dose-response curve of low density lipoprotein peroxidation induced by Cu2+shifted to the left in this group as compared with the curve in the other three groups. The vitamin E (alphatocopherol) content of low density lipoprotein fraction in these patients was significantly lower than that in the other groups. After ≥6 months of an angina-free period in five patients with active variant angina, thiobarbituric acid—reactive substances induced by 0.5 μmol/liter Cu2+in low density lipoprotein were significantly decreased and vitamin E content was significantly increased.
Conclusions These results indicate that vitamin E-deficient plasma low density lipoprotein in patients with active variant angina is highly susceptible to peroxidative modification.
This study was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, Tokyo, Japan.
- Received July 1, 1994.
- Revision received January 12, 1995.
- Accepted April 7, 1995.
- American College of Cardiology