Author + information
- Received April 20, 1995
- Revision received July 7, 1995
- Accepted August 10, 1995
- Published online January 1, 1996.
- Abraham Rothman, MD, FACC**,a,
- David M. Mann*,
- Mark T. House*,
- Ron G. Konopkaa,
- Peter G. Chilesa,
- Craig A. Pedersena,
- Paul Wolf, MDa and
- Kenneth M. Moser, MDa
- ↵**Address for correspondence: Dr. Abraham Rothman, University of California San Diego Medical Center, 8445, 200 West Arbor Drive, San Diego, California 92103.
Objectives. The aim of this study was to evaluate the performance of a new arterial biopsy catheter in obtaining pulmonary endovascular samples in a canine model.
Background. Percutaneous endomyocardial biopsy is a widely used and valuable procedure in the management of posttransplant rejection and selected cardiomyopathies. A similar method of obtaining endoarterial biopsy samples would aid in the study, diagnosis and management of arterial diseases.
Methods. Catheterization was performed in 19 dogs, each weighing 20 to 30 kg, through an 8F sheath in the external jugular vein to obtain pulmonary endoarterial samples. The catheter consists of two sliding tubes: an inner one with a beveled opening that accommodates endoarterial tissue by means of a vacuum and an outer tube with a sharp distal edge that cuts the tissue when activated.
Results. Overall, a total of 266 separate biopsy attempts were performed, and 161 tissue samples were obtained (success rate 61%). With modifications in technique in the last nine dogs, 54 (93%) of 58 attempts were successful. There were no deaths, extravasation of contrast material on angiography or thrombi. Of 20 vessels with prebiopsy and postbiopsy angiograms, 1 developed transient spasm (5%). On microscopic examination of cross sections of 50 separate pulmonary endoarterial biopsy samples, all had smooth muscle cells and 30 contained endothelial cells (60%). The arteries of origin showed small intimal and medial tears and mild perivascular hemorrhage. Angiographic and pathologic examination of previously biopsied arterial segments 2 weeks (two dogs) and 8 weeks (two dogs) after the procedure showed patent vessels and no thrombi. Histologically, the biopsy sites revealed mild neointimal and medial proliferation.
Conclusions. This new endoarterial biopsy catheter is safe and effective in obtaining pulmonary artery samples in normotensive dogs.
- Received April 20, 1995.
- Revision received July 7, 1995.
- Accepted August 10, 1995.
- American College of Cardiology