Identification of bone matrix markers in calcified human rheumatic valves. Stains as in Figure 2. C = osteocalcin immunostain. Arrowpoints to myofibroblast staining cells (magnification ×40). All others, magnification ×10. Micro CT 3D reconstruction of calcified rheumatic valve (D1) and uncalcified degenerative mitral valve (D2). From Rajamannan et al. (4). Prints of figure provided by N.M. Rajamannan, MD.