Author + information
- Martin Rodriguez-Porcel, MD,
- Todd J. Brinton, MD,
- Ian Y. Chen, MSE,
- Olivier Gheysens, MD,
- Jennifer Lyons,
- Fumiaki Ikeno, MD,
- Jürgen K. Willmann, MD,
- Lily Wu, MD, PhD,
- Joseph C. Wu, MD, PhD,
- Alan C. Yeung, MD,
- Paul Yock, MD and
- Sanjiv Sam Gambhir, MD, PhD⁎ ()
- ↵⁎Stanford University School of Medicine, Bio-X Program, Departments of Radiology and Bioengineering, The James H. Clark Center, 318 Campus Drive, Clark E150, Stanford, California 94305-5427
To the Editor: Noninvasive monitoring of cardiac gene therapy is critical to fully understand the biology of gene therapy in living subjects. We and others have monitored reporter gene expression in the myocardium of small (1) and large (2) animals (reviewed in reference 3). However, before these strategies are translated to the clinic, it is critical that they be tested using minimally invasive gene delivery approaches similar to those used clinically.
We tested the hypothesis that reporter genes can be delivered using a minimally invasive strategy to the myocardium of a swine, and expression can then be imaged using combined positron emission tomography-computed tomography (PET-CT).
Stanford’s Animal Care and Use Committee approved all procedures. Six domestic pigs (Pork Power Farms, Turlock, California) were used in the study. With sterile technique, 8-F vascular sheaths placed in the carotid arteries were used for vascular access. Percutaneous endomyocardial delivery systems (Biocardia, Inc., South San Francisco, California) (Fig. 1, left panel), placed into the sheaths, were used for fluoroscopy-guided delivery of genes (1010 plaque-forming units) or vehicle (phosphate-buffered saline [PBS]) (Fig. 1, right panel) in 3 doses of 0.2 cc each. Central mean arterial pressure (MAP) was measured. Forty-eight hours after gene delivery, animals were dynamically imaged after intravenous administration of 18F-labeled 9-[4-fluoro-3-(hydroxymethyl)butyl]guanine (18F-FHBG; tracer) (4) using a clinical combined PET-CT system (Discovery LS, GE Medical Systems, Milwaukee, Wisconsin) for a total scanning time of 180 min. Data are expressed as mean ± SEM.
There were no significant differences in weight (control, 37.4 ± 0.4 kg; gene therapy, 36.3 ± 0.7 kg; p = NS), MAP (control, 107 ± 11 mm Hg; gene therapy, 111 ± 14 mm Hg; p = NS), or heart rate (control, 90 ± 7 beats/min; gene therapy, 95 ± 9 beats/min; p = NS) between the 2 groups. There was no morbidity or mortality associated with the procedures. A total of 9.37 ± 1.31 mCi of 18F-FHBG (in 5 ml of PBS) was administered per animal.
Figure 2 (top panel, A to D) shows a representative PET-CT scan of the gene therapy group. The CT images (Fig. 2A, top panel) were used for anatomic localization, and 18F-FHBG uptake (Fig. 2B, top panel) was located in the area into which gene therapy was delivered (anteroseptum) (Fig. 2C, top panel). Whole-body images (Fig. 2D, top panel) clearly showed the cardiac uptake in chest and abdominal structures.
Animals from both groups had comparable 18F-FHBG uptake in paraspinal muscles and the nondelivered myocardial wall (Figs. 2E and 2F). Whereas control animals showed no distinct myocardial tracer uptake, experimental animals had significantly increased (p < 0.05) 18F-FHBG uptake (Figs. 2E and 2F, respectively). The best myocardial signal/background (left ventricular) ratio was obtained 3 h post-injection (180 min, 4.63 ± 1.4 vs. 90 min, 1.78 ± 0.6; p < 0.05). Autoradiography and microPET confirmed the increased 18F-FHBG uptake in the anteroseptum of the gene therapy animals.
Many different delivery methods have been developed for percutaneous cardiac delivery of gene therapy. The helical needle injection catheter system, used in this study, has the theoretic advantages of endocardial engagement and helical needle-track and has been shown to have good acute delivery success and retention (5). This delivery method has been designed to deliver material (e.g., genes, cells) to a specific and delimited area. Multiple injections or vascular-based delivery methods (e.g., intracoronary) may be more useful if the target area is a larger myocardial region or a specific coronary distribution.
Adenoviral infection results in strong, albeit relatively short-lived, transgene expression (6). For performing long-term longitudinal monitoring of therapy, other reporter gene strategies will be needed, such as adeno-associated or gutless adenovirus (7,8).
PET has nanomolar to picomolar (10−12 mol/l) sensitivity and tomographic capabilities, which makes PET the most suitable imaging modality for use in living subjects (compared with magnetic resonance and single photon emission-computed tomography) (9). Based on this study, 3 h after tracer administration appears to be a good time point for assessment of 18F-FHBG uptake in the myocardium.
These studies will play a critical role in the monitoring of gene therapy first in pre-clinical large animal models of cardiac disease and then in clinical therapeutic trials.
Please note: this study was supported by grants NCI SAIRP (to Dr. Gambhir), NHLBI R01 HL078632 (to Dr. Gambhir), NCI ICMIC CA114747 P50 (to Dr. Gambhir), NHLBI R21HL089027 (to Dr. Wu), and the Mayo Clinical Scholarship Program, Mayo Clinic College of Medicine, Rochester, Minnesota (to Dr. Rodriguez-Porcel). The authors thank Olin Palmer and Daniel Rosenman from BioCardia, Inc., for their technical assistance with the catheter delivery system and the Stanford cyclotron team (Dr. Fren Chin and Dr. David Dick) for 18F-FHBG production.
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