Author + information
- Received February 21, 2008
- Revision received August 26, 2008
- Accepted September 4, 2008
- Published online March 31, 2009.
- Ronen Jaffe, MD*,†,§,
- General Leung, MSc‡,§,
- Nigel R. Munce, MSc‡,§,
- Amandeep S. Thind, BSc‡,§,
- Howard Leong-Poi, MD†,§,
- Kevan J.T. Anderson, BSc‡,§,
- Xiuling Qi, PhD, MD‡,
- Judy Trogadis, BSc†,
- Ariella Nadler, BA*,
- Davida Shiff*,
- Jamie Saperia*,
- Julia Lockwood*,
- Chaim Jacobs*,
- Beiping Qiang, MD*,†,
- Aaron Teitelbaum, MD, MSc*,
- Alexander J. Dick, MD*,§,
- John D. Sparkes, MSc*,
- Jagdish Butany, MD§∥,
- Graham A. Wright, PhD*,‡,§ and
- Bradley H. Strauss, MD, PhD*,§,* ()
- ↵*Reprint requests and correspondence:
Dr. Bradley H. Strauss, Reichmann Chair in Cardiovascular Sciences, Sunnybrook Health Sciences Centre, 2075 Bayview Avenue, Room A-253, Toronto, Ontario, Canada M4N 3M5
Objectives We sought to perform the first systematic study of the natural history of chronic total arterial occlusions (CTOs) in an experimental model.
Background Angioplasty of CTOs has low success rates. The structural and perfusion changes during CTO maturation, which may adversely affect angioplasty outcome, have not been systematically studied.
Methods Occlusions were created in 63 rabbit femoral arteries by thrombin injection. Histology, contrast-enhanced magnetic resonance imaging, relative blood volume (RBV) index, and micro-computed tomography imaging were analyzed at 2, 6, 12, and 18 to 24 weeks.
Results Early changes were characterized by an acute inflammatory response and negative arterial remodeling, with >70% reduction of arterial cross-sectional area (CSA) from 2 to 6 weeks. Intraluminal neovascularization of the CTO occurred with a 2-fold increase in total (media + intima) microvessel CSA from 2 to 6 weeks (0.014 ± 0.002 mm2to 0.023 ± 0.005 mm2, p = 0.0008) and a 3-fold increase in RBV index (5.1 ± 1.9% to 16.9 ± 2.7%, p = 0.0008). However at later time periods, there were significant reductions in both RBV (3.5 ± 1.1%, p < 0.0001) and total microvessel CSA (0.017 ± 0.002 mm2, p = 0.011). Micro-computed tomography imaging demonstrated a corkscrew-like recanalization channel at the proximal end at 6 weeks that regressed at later time points. These vascular changes were accompanied by a marked decrease in proteoglycans and accumulation of a collagen-enriched extracellular matrix, particularly at the entrance (“proximal fibrous cap”).
Conclusions This study is the first to systematically analyze compositional changes occurring during CTO maturation, which may underlie angioplasty failure. Negative remodeling, regression of intraluminal channels, and CTO perfusion, together with the accumulation of dense collagen, may represent important targets for novel therapeutic interventions.
Dr. Jaffe is a Research Fellow of the Heart and Stroke Foundation of Canada. This study was funded by the Canadian Institute of Health Research (MOP-53325, CTP-82943, and MOP-12492).
- Received February 21, 2008.
- Revision received August 26, 2008.
- Accepted September 4, 2008.
- American College of Cardiology Foundation