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Morphological data of thrombus responsible for STEMI is derived from autopsy with its inherent limitations. A detailed compositional analysis of the ex vivo thrombi aspirated during primary PCI in patients with STEMI is lacking.
Thrombectomy specimen from 15 patients with STEMI were subjected to serial 5 micron cryotomy, immuno-stained using a panel of antibodies for platelets (CD42a-PerCP, CD41a-PerCP, PAI-1-FITC, leukocytes (DAPI, CD14- PE-Cy5, CD11b-PE-Cy5, CD3-FITC, CD20-FITC, Vimentin-Alexa Fluor-647, Fibrin (FITC). The sections were analysed with a 3-color immuno-microscope and cells quantified by measuring the intensity the specific fluorphore. 10 high power fields were analysed in each section. Using a grid provided in the software the total number of leucocytes and their subpopulation were counted.
Distinct polarization of the distribution of platelets, leukocyte and fibrin was observed. The granulocytes (DAPI+ CD14- CD3-/ CD20-) accounted for 89.5 % of the leucocytes. The monocytes (DAPI+ CD14+) accounted for 10.5 % of the leukocytes. CD3+ was only very scarcely present and no CD20+ B lymphocytes were observed. The leykocytes intensely expressed CD11b (MaC-1) and vimentin.
Heterogenously distributed aggregates platelets and leukocyet were the integral component of thrombus. Since CD11b can function as a fibrin receptor, its expression on thrombi leukocytes suggest that it may play import role in thrombus formation and stabilization.
Poster Sessions, Expo North
Sunday, March 10, 2013, 9:45 a.m.-10:30 a.m.
Session Title: Acute Coronary Syndromes: Basic III
Abstract Category: 2. Acute Coronary Syndromes: Basic
Presentation Number: 1213-190
- 2013 American College of Cardiology Foundation