Author + information
- Harsha Kannan,
- Eleonora Mezzaroma,
- Rossana Bussani,
- Stefano Toldo,
- Benjamin Van Tassell,
- Furio Silvestri,
- Alfonso Baldi,
- Gianfranco Sinagra and
- Antonio Abbate
Acute myocarditis is characterized by myocardial damage and dysfunction often due to a viral infection, and followed by a variable recovery. The inflammasome is a recently identified macromolecular structure that amplifies the inflammatory response and promotes cell death. Whether the inflammasome is formed in acute myocarditis is unknown.
In this study we included 8 patients with biopsy-documented acute myocarditis considered to be likely viral in origin, and 8 cases in which the diagnosis of acute myocarditis was made post-mortem. Formation of the inflammasome was detected using immunofluorescence for the apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC), the scaffold protein that appears as an aggregate (speck) during inflammasome formation.
The inflammasome was detected in 6 of 8 cases (75%) of biopsy-proven myocarditis as well as in 6 of 8 cases (75%) of post-mortem diagnosis. A median of 2 inflammasome specks (interquartile range 1-5) per high power field (HPF, x40) were seen. Two cases of showed intense staining for the inflammasome, mostly in the leukocytes (40-50 per HPF). The figureshows mild [left panel] and intense [right panel] staining for ASC (red) paired with cardiac actin (green) and DNA staining (blue).
Acute myocarditis is characterized by a variable formation of the inflammasome in the myocardium. The role of the inflammasome in myocardial healing during acute myocarditis remains to be defined.
Poster Sessions, Expo North
Sunday, March 10, 2013, 9:45 a.m.-10:30 a.m.
Session Title: Myocarditis: Mechanistic Insights
Abstract Category: 23. Pericardial/Myocardial Disease
Presentation Number: 1207-146
- 2013 American College of Cardiology Foundation