Author + information
- Chiara Giannarelli,
- David T. Rodriguez,
- Claudia Calcagno,
- Randolph Hutter,
- Antoine Millon,
- Peter Faries,
- Zahi Fayad,
- Roger Hajjar,
- Valentin Fuster and
- Juan Badimon
Alternatively Spliced Tissue Factor (asTF) is a novel isoform of human Tissue Factor (TF) exhibiting angiogenic activity. TF is highly expressed in unstable plaques; however, asTF expression in atherosclerosis and its importance in plaque instability remain unknown.
Carotid plaques (n=10) were obtained from Institutional Bio-Bank. Coronary endarterectomy specimens were collected from patients with stable angina (n=4) or acute coronary events (ACS/AMI; n=4). All plaques were classified according to the AHA classification. asTF, TF, macrophage (CD68+) and neovessels (CD31+) were assessed by immunohistochemistry.
Carotid plaques were identified as Type IV-V (lipid-rich with fibrotic cap, non-hemorrhagic; n=5) and type VI (disruption, hemorrhage; n=5). Clinically stable coronary plaques were classified as type V plaques (n=4) while unstable (ACS/AMI) ones as type VI (n=4). Type VI plaques showed a higher asTF and TF expression than type IV-V (Fig1A). Increased expressions of asTF, TF, CD68+ and CD31+ cell density were detected in type VI vs. type V coronary plaques. A strong co-localization of asTF with macrophages and neovessels was observed in unstable plaques (Fig. IB).
asTF is highly expressed in unstable carotid and coronary plaques. asTF appears to be specifically expressed by a subset of endothelial cells and macrophages in clinically unstable coronary plaques. These data suggest a major role for asTF in plaque instability and clinical events.
Poster Sessions, Expo North
Sunday, March 10, 2013, 9:45 a.m.-10:30 a.m.
Session Title: Acute Coronary Syndromes: Basic III
Abstract Category: 2. Acute Coronary Syndromes: Basic
Presentation Number: 1213-192
- 2013 American College of Cardiology Foundation