Author + information
- Georgia Vogiatzi,
- Dimitris Tousoulis,
- Alexandros Briasoulis,
- Aggeliki Valatsou,
- Anna–Maria Kampoli,
- Charalambos Antoniades,
- Christina Chrysochoou,
- Kostas Toutouzas,
- Costas Tentolouris,
- Despoina Perrea and
- Christodoulos Stefanadis
Bone marrow derived progenitor cells have been suggested to promote postnatal neovascularization. In this study we investigated whether direct intramuscular infusion of enriched hematopoietic cells, improved limb perfusion in a murine model of hind limb ischemia.
Wild type C57BL/6 male mice underwent unilateral hind–limb ischemia, were divided in three groups (n=12/group) and received a single intramuscular injection of 1×106 Lin–/sca+ cells, or granulocyte colony–stimulating factor (G–CSF) for 7 days or normal saline. Each group mice underwent Laser Doppler perfusion Imaging on days 1, 7 and 28 after surgery for the estimation of the bilateral hind–limb perfusion. At day 28 they were sacrificed and quantitative real time RT–PCR was performed to the muscle tissues from both limbs to analyze the differential gene expression of vascular endothelial growth factor (VEGF), Tie–2, Ang–1 and Ang–2. Muscle tissue sections were stained with rat anti–CD31antibody. Capillaries and arterioles in the ischemic areas were counted with confocal microscopy at day 28.
Ischemic/non ischemic ratio was significantly increased in ischemic limbs of cell– and G–CSF–treated mice versus control mice at 7 days (p<0.05 vs control), which was maintained at 28 days (p<0.05 vs control) only in the cell–treated group. There was no significant increase of ischemic/nonischemic ratio in the cell–treated mice compared with G–CSF at day 7 or day 28 (p=NS). Capillary density was increased in the cell–treated group compared to G–CSF–treated group and control (p<0.05). No difference in the capillary density between the G–CSF–treated and the control group was observed. Compared to the G–CSF and control group, the expression of VEGF (p<0.05), Ang–2 (p<0.05) and sTie–2 (p<0.05) were significantly increased in the ischemic limbs of the Epo–treated group. In contrast, the Ang–1 expression didn't significantly differ between the three groups.
Direct intramuscular infusion of lin–/sca+ significantly improves blood flow and increases neoangiogenesis by upregulation of the Ang–2/Tie–2 pathway when compared with G–CSF and control treatment in ischemic limbs.
Moderated Poster Contributions
Poster Sessions, Expo North
Sunday, March 10, 2013, 3:45 p.m.–4:30 p.m.
Session Title: Cell Therapy and Angiogenesis
Abstract Category: 42. TCT@ACC–i2: Cell Therapy & Angiogenesis
Presentation Number: 2110M–227
- 2013 American College of Cardiology Foundation