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We previously reported that amniotic mesenchymal stem cells (AMMs) possess high angio–vasulogenic properties. In this study, we investigated the chemotactic abilities of AMMs for improved cardiac function and regenerative angiogenesis.
The expressions of chemotactic and angiogenic genes were determined by qRT–PCR. Myocardial infarction (MI) was induced in NOD/SCID mice and cells were directly transplanted into the border regions of ischemic heart tissue. Immunohistochemical analysis was also conducted.
AMMs significantly expressed the representative chemotactic factor GCP–2, NAP–2 as well as angiogenic factor Hif–1a. AMMs also highly expressed the chemokine receptors CCR2, CCR3 and CCR5. AMM transplantation improved left ventricular function, capillary density, angiogenic cytokine levels, angiopoetin (Ang)–1 and vascular endothelial growth factor (VEGF–A) levels in affected tissue. Immunohistochemical assaying also revealed increased engraftment and endothelial phenotypes.
Our findings suggest that due to elevated survival and related chemotactic potential, AMMs are a promising stem cell source for the treatment of ischemic cardiovascular disease.
Poster Sessions, Expo North
Sunday, March 10, 2013, 3:45 p.m.–4:30 p.m.
Session Title: Angiogenesis and Vascular Injury
Abstract Category: 33. Vascular Medicine: Basic
Presentation Number: 1254–170
- 2013 American College of Cardiology Foundation