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Circulating microparticles (CMPs) and cell free circulating DNA (CFC-DNA) have previously been shown to be elevated in the setting of acute myocardial infarction (MI). To date, measurement of such markers has been limited to the research arena. These biomarkers may also reflect atherosclerotic plaque rupture rather than solely myocardial necrosis. Using a novel, whole blood based, point of care alternating current device (ACE Chip) these biomarkers can be measured rapidly as a potential diagnostic marker of acute atherosclerotic plaque rupture.
4ml of whole blood was collected from 19 MI samples and 17 healthy controls. Within the ACE Chip the sample is spiked with SYBR Green 1 dye and green fluorescence imagery is captured after isolation. 100–200uL of plasma is then run and the particles counted. To assess the endothelial origin of these particles the protein biomarkers CD 146, 31, 33, 34 and 105 were tested. Data was analyzed using a non-parametric Mann-Whitney U test.
The mean CMP/ml in MI patients was 1062 CMPs/ml versus 42 CMPs/ml in healthy controls (p<0.001). An optimized cut off point of 52 CMPs/ml demonstrates a sensitivity of 95% and specificity of 82%. Isolated CMPs stained positive for CD31, CD 33 and CD 105 but were negative for CD 34 and CD146.
CMPs can be measured using a novel point of care electrical isolation device and are present at greater levels in those with MI as compared to healthy controls. These microparticles may prove a valuable marker of plaque rupture preceding myocardial necrosis in MI.
Moderated Poster Contributions
Poster Sessions, Expo North
Saturday, March 09, 2013, 3:45 p.m.-4:30 p.m.
Session Title: Acute Coronary Syndromes: Basic II
Abstract Category: 2. Acute Coronary Syndromes: Basic
Presentation Number: 1169M-178
- 2013 American College of Cardiology Foundation