Author + information
- Ayşe Yiğit1,
- Efkan Uz2,
- H. Ramazan Yılmaz3,
- Abdullah Doğan4,
- Barbaros Yiğit5,
- Atilla İçli6 and
- Habil Yücel7
Glutathione S transferases (GSTs) are enzymes responsible for the metabolism of numerous xenobiotics and are known to be polymorphic in humans. Glutathione S-transferases (GSTs) detoxify environmental agents which influence the onset and progression of disease. Carcinogenic and toxic molecules produce DNA adducts that contribute to the development of atherosclerosis. Genetic polymorphisms of xenobiotic-detoxified enzymes, which control the level of DNA adducts, may affect both enzymatic activity and individual susceptibility to coronary artery disease (CAD). Dysfunctional detoxification enzymes are responsible for prolonged exposure to reactive molecules and can contribute to endothelial damage, an underlying factor in CAD. Coronary artery ectasia (CAE) is defined as local or generalized aneurysmal dilatation of the coronary arteries. Although the etiology of CAE has not been identified completely, the most frequent cause is coronary atherosclerosis. Endothelial dysfunction and enzymatic destruction of media layer plays an important role in the pathogenesis of coronary ectasia. We aimed to examine the association between GSTM1, T1 gene polymorphisms and CAE.
Our study was carried out in a total of 90 cases diagnosed with CAE and a total of 91 population-matched healthy controls in respect to age and genders. After DNA isolation, polymorphisms were analyze using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. Distribution of the GSTM1 and GSTT1 polymorphism genotypes were determinated as Wild (*1*/1) genotype (Normal genotype) and null (*0*/0) genotype (homozygous deletion). In our study CAE group was also divided into subgroups in respect to herbicide exposure.
No significant differences were obtained in terms of age, genders, risk factors, biochemical parameters and echocardiographic characteristics between study and control groups. The levels of GSTM1 and T1 polymorphisms were statistically higher in CAE cases than controls (p<0,05). Genotype distribution and the levels of GST enzymes of CAE and control groups shown in the table. GSTM1 and T1 homozygous mutations were detected significantly higher in those with exposure to herbicides in patients with coronary artery ectasia group.
To our knowledge this is the first study examining the polymorphisms encoding GST enzymes polymorphisms in cases with CAE. Our results suggest that the deletions in GST genes associated with patients CAE. We think the data obtained in this study can be used to direct people who have deletions in GST genes to avoid chemicals and herbicide exposure.