Author + information
- Huseyin Altug Cakmak1,
- Hasan Ali Barman1,
- Ender Coskunpinar2,
- Yasemin Musteri Oltulu2,
- Baris Ikitimur1,
- Gunay Can3,
- Sevgi Ozcan1 and
- Vural Ali Vural1
MicroRNAs (miRNAs) are small, endogenous and non-coding RNAs that are 21-25 nucleotides in length. Recent studies have demonstrated the role of miRNAs as potential biomarkers in various disorders. The aim of this study was to investigate the diagnostic importance of miRNAs in congestive heart failure (CHF).
In our study, 20 clinically stable (NYHA II) and 22 decompansated (NYHA III and IV) systolic CHF (EF<%40) patients and 15 healthy controls were enrolled. Whole genome miRNA profiling was done and dysregulated miRNAs were detected with microarray method in CHF patients compared to healthy controls. Also, the serum levels of pro-Brain Natriuretic Peptide (BNP), high sensitive C-reactive protein (hs-CRP) and uric acid were measured.
Microarray profiling showed serum miR-21, miR-4278, miR-650, miR-744star, miR-516-5p, miR-1292, miR-182, miR-1228, miR-595, miR-663b, miR-1296, miR-1825, miR-299-3p, miR-662 miR-122-star, miR-3148, miR-518e-star, miR-2054 as up regulated, and serum miR-129-3p, miR-3155, miR-3175, miR-583, miR-568, miR-30d, miR-200a-star, miR-1979, miR-371-3p, miR-155-star, miR-502-5p as down regulated in CHF.
The diagnostic values of miR-4278 (AUC 1.00 p<0.0001), miR-516-a-5p (AUC 1.00 p<0.0001) and miR-1228 (AUC 1.00 p<0.0001) were found to be very high compared to other known important clinical prognostic markers as pro-BNP (AUC 0.98 p<0.0001), hs-CRP (AUC 0.958 p<0.0001) and uric acid (AUC 0.97 p<0.0001) with ROC curve analysis in patients with CHF (Figure).
We identified 29 miRNAs that were elevated in patients with CHF, among which miR-4278, miR-516-a-5p and miR-1228 were most significant in terms of clinical diagnosis. This study demonstrates the value of miR-4278, miR-516-a-5p, miR-1228 as potential diagnostic biomarkers in CHF.