Author + information
Ischaemia /reperfusion induce myocyte necrosis and apoptosis that seem to be the prevalent mode of death during the ischemic period and the subsequent reperfusion. L-carnitine is essential for the transport and metabolism of fatty acids to the mitochondria for energy production.
Aim of the study
We aimed to study the possible cardio protective potential of L-carnitine in myocardial regional ischemia – reperfusion injury.
Dwale-sprague rats were divided into five groups (6 rats for each) assigned as I, II, III, IV, V. Group I (sham), rats were subjected for all surgical procedure without ligation of (LAD). GroupII (control), rats were subjected for entire surgical procedure with ligation of (LAD). GroupIII (control vehicle), rats pretreated with normal saline (vehicle for L-carnitine) for 7days then subjected for entire surgical procedure with ligation of (LAD). Group (L-carnitine treated group), rats previously treated with (100 mg/kg, IP) L-carnitine then subjected to entire surgical procedure including LAD ligation for 25 minutes followed by 120 minutes reperfusion. At the end of reperfusion,cardiac tissue TNF-α, IL-1β, IL-6 and ssDNA as well as plasma cardiac troponin I (cTnI) were measured.It has been found that L-carnitine treated group showed significant reduction (ρ<0.05) in TNFα, IL-1β, IL-6, ssDNA (a marker of apoptosis) and cTnI with respect to the control or control vehicle groups. Additionally histopathological study revealed that L-carnitine significantly (ρ<0.05) improved cardiac injury score as compared with control group. It is concluded from this study that L-carnitine reduce inflammatory reaction associated with ischemia/reperfusion and ameliorates apoptosis.