Author + information
- Yan Wenwen and
- Lemin Wang
To evaluate the natural innate and adaptive immunity through gene expression and cytology levels in peripheral blood mononuclear cells in patients with acute myocardial infarction (AMI), stable angina pectoris (SAP) and the controls.
210 patients with AMI, 210 with SAP, and 250 clinical controls were recruited into the study. 20 randomly chosen subjects per group were examined for the whole human genome microarray analysis to detect the expressions of intracellular and extracellular markerfor complement, CD16+CD56+ natural killer cells, CD3+, CD4+, CD8+T cells and CD19+B cells. The quantity of these cells and related cytokines and immunoglobulin levels were measured in all 670 subjects.
1) Gene expression level: In AMI group, the mRNA expressions of late complement component, the markers of natural killer cells, CD3+, CD8+ T cells and B cells were significantly down-regulated, while early complement component and CD4+T cell expressions were significantly up-regulated (p < 0.05)when compared with SAP and the controls. 2) Cytology levels: In both AMI and SAP patients, the quantity of natural killer cells,CD3+, CD8+ T cells, B cells, IgM and IgG were significantly lower than those of the controls. On the contrary CD4+ T cells, CH50, C3, C4, IL-2, IL-4, IL-6 and IFN-γ were significantly higher (p < 0.05).
In AMI patients, both of gene expressions related to complement, natural killer cells, CD3+, CD8+ T cells, B cells and the quantity of these immune cells decreased simultaneously while only cell number reduced in SAP patients. It clearly indicated that the immune system function gradually weakened in AMI and SAP patients. To improve the immune system may be considered as a potential target for medical interventions and prevention in AMI.