Author + information
- Bao Zhengyang,
- Ding Ying-peng,
- Wang Zhong-qun,
- Yan Jin-chuan and
- Wang Zhong-qun
To investigate the effect of ghrelin induces migration via PI3K/Akt signaling pathway in foam cells.
RAW264.7 macrophages were cultured in DMEM/LOW GLUCOSE, supplemented with 10% fetal bovine serum for 24 h. RAW264.7 macrophages were randomly assigned into four groups: control group (RAW264.7 macrophages), oxLDL group (RAW264.7 macrophages + oxLDL), ghrelin group (RAW264.7 macrophages + oxLDL + ghrelin) and ghrelin inhibitor group (RAW264.7 macrophages + oxLDL + ghrelin + LY294002). Total cholesterol, free cholesterol and cholesterol ester measurement, oil red Ostaining,transwell insert, semi-quantitative Western blot analysis,immunofluorescence staining were then performed.
Oil red O staining suggested that oxLDL could dramatically induce Raw264,7 macrophages transdifferentiation into foam cells. Cholesterol oxidase method, transwell insert and immunofluorescence staining demonstrated that ghrelin significantly promote the migration of RAW264.7 macrophages (p<0.05), whereas it could be reversed by ghrelin inhibitor LY294002 (p<0.05). Semi-quantitative Western blot analysis indicated that ghrelin could efficiently up-regulate the expression of p-Akt but down-regulate the content of cleaved caspase-3 (p<0.05), similarly, LY294002 reversed the changes of the above indexes (p<0.05).
Ghrelin could promote the migration of RAW264.7 derived foam cells, which may be related to the activation of the PI3K/Akt cellular pathway.