Author + information
- Wang Huan and
- Chen Hui
Circadian rhythm disruption is shown to be the cause of various health disorders. Circadian Locomotor Output Cycles Kaput (CLOCK) and brain and muscle Arnt-like protein-1 (BMAL1), two core circadian transcriptionfactors, were associated with the regulation of glucose homeostasis. This study evaluated the association between single nucleotide polymorphisms (SNPs) in CLOCK and BMAL1 gene and insulin resistance (IR) in hypertensive patients.
We collected 334 outpatients with essential hypertension (EH), who have not taken any antihypertensive agents or stopped the medications for at least a week for this case-control study. The value of homeostasis model assessment of insulin resistance (HOMA-IR) more than 2.69 was considered as IR positive. Clock T3111C & Bmal1 A1420G genotypes were determined by polymerase chain reaction and automated DNA Sequencer using peripheral blood DNA as a template.
A total of 334 patients were analyzed (103 in the HOMA-IR positive group and 231 in the HOMA-IR negative group). Compared with the HOMA-IR negative group, waist circumference, body mass index (BMI), glycated hemoglobin (HbA1C), total cholesterol, triglyceride (TG) and plasminogen activator inhibitor-1 were significantly increased, while high-density lipoprotein cholesterol (HDL-C) was significantly decreased in the HOMA-IR positive group (P<0.05-0.001). The results of 24-hours ambulatory blood pressure monitoring showed that 24-hours mean systolic blood pressure (SBP), especially the night SBP was higher in the HOMA-IR positive group (P<0.05). Notably, the distribution frequency of the C allele of Clock T3111C and the GG genotype of Bmal1 A1420G were significantly higher in the HOMA-IR positive group (29.1vs 10.8%, p<0.000 and 43.7vs 27.7%, p =0.007). We found a strong association between the absence of the C allele of Clock T3111C (odds ratio [OR]=4.128, CI 95% 2.313-7.368, p=0.000), as well asthe GG genotype of Bmal1 A1420G (OR=1.983, CI 95% 1.117-3.521, p=0.019), and the increased HOMA-IR in Chinese EH patients.
Hypertensive patients who carried the C allele of Clock T3111C or the GG genotype of Bmal1 A1420G might be susceptibility to IR, which could be associated with elevated night SBP.