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This study aimed to evaluate the role of Arg389Gly ADRB1 gene polymorphism in chronic heart failure (CHF) manifestation in Uzbek patients.
The study involved 154 CHF (ischemic genesis) male patients, Uzbeks, aged from 41 to 60 years. The control group comprised 157 healthy, age-matched, randomly selected male persons. All the patients were divided into three groups according to the New York Heart Classification (NYHA) functional class (FC). Genomic DNA was extracted from peripheral blood using the Diatomtm DNA Prep 200 Kit according to the manufacturer's protocol. Polymerase chain reaction restriction fragment length polymorphism-based (PCR-RFLP) techniques.
The analysis of distribution of Arg389Gly ADRB1gene polymorphism in CHF patients showed: a genotype Arg/Arg occurred at 62.3%, a genotype Arg/Gly - in 33.1% and Gly/Gly - in 4.5% of patients. A genotype Arg/Arg in patients with CHF FC I was 48.1%, patients with FC II have 58.6% and FC III- 71.0%. Arg/Gly genotype occurred in 11 patients of FC I - 40.7%, 23 patients of FC II - 39.6% and in 17 patients with CHF FC III - 24.6%. In group of healthy people Arg/Arg genotyping occurred in 46.5%, Arg/Gly in 31.2%. In patients of control group occurrence of Arg alleles was 80.6% and Gly alleles - 19.4%. In CHF patients a frequency of distribution of Arg alleles was 78.9% and Gly -alleles - 21.1%.
Thus, studying of distribution of alleles and genotypes of Arg389Gly ADRB1 gene showed that in both CHF patients and healthy people of the Uzbek nationality prevailed Arg/Arg genotypes and Arg alleles.