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This aim of this study was to measure the mRNA and protein expression levels of VAMP2 and observe the difference of VAMP2 level between the patients with chronic atrial fibrillation and the patients with sinus rhythm.
The protocol of this study was approved by Ethics Review Board of Southwest Medical University. All of the procedures were done in accordance with the Declaration of Helsinki and relevant policies in China. Specimens of right atrial appendage were obtained from patients who accepted cardopulmonary bypass. There were no statistical differences in age and gender. VAMP2 mRNA level was obtained from 12 patients with sinus rhythm (SR group) and 13 patients with chronic atrial fibrillation (AF group). The protein extractions were detected by western blotting. VAMP2 protein level was obtained from 15 patients with sinus rhythm (SR group) and 14 patients with chronic atrial fibrillation (AF group). The expression of mRNA were detected by quantitative real-time PCR. The data was analyzed with 2-ΔΔCT method. The statistical analysis was done using independent-samples T test by SPSS 17.0.
The mRNA level of VAMP2 in atrial tissue from patients with AF was increased significantly, compared with that from SR patients (P < 0.01). It was consistent with the change in mRNA level, the protein expression of VAMP2 was also increased in patient atrial tissue with AF (P < 0.01).
The mRNA and protein expression level of VAMP2 was significantly up-regulated in patients with chronic atrial fibrillation. It may demonstrate that VAMP2, an important SNAREs protein, modulated the trafficking of intracellular ion channel proteins and involved the translocation of ion channels in AF.