Author + information
- Li Wenhua
To observe the effect of curcumin (CUR) intervention on contrast-induced acute kidney injury in experimental rats, and to explore the beneficial effects and the possible mechanism of curcumin intervention on acute kidney injury in experimental rats by the low non-ionic contrast medium, in order to provide more theoretical basis for treating such injury in clinical practice.
A total of 30 male SD rats were randomly divided into 3 groups: control group, contrast-induced nephropathy group and curcumin group, ten of each group. The rats of curcumin group received intra-gastric curcumin for 5 days, the other two groups were given the corresponding volume of solvent for 5 days. After 48 hours of building model, The renal function was evaluated by serum creatinine(Scr) and urea nitrogen(BUN), the pathological change of the renal tissue was observed by HE staining to evaluate the degree of renal tissue injury, the nephridial oxidative stress indexes of malonadehyde (MDA) level and super oxide dismutase (SOD) activity were measured by the operating kits, then the protein expression of Sirt1 and NF-κB was examined by westernblot.
Compared with control group, contrast-induced nephropathy group had significantly increased levels of Scr, BUN, MDA, NF-κB and decreased SOD activity, Sirt1 level, respectively P<0.01; respectively the HE staining of the rats in contrast-induced nephropathy group showed severe tubular injure, medullary congestion, obvious tubular structure damage, epithelial cell brush border loss, vacuolar degeneration, necrosis and protein deposition, and had increased scores of tubular injure, P<0.05. Compared with contrast-induced nephropathy group, curcumin group had obviously decreased levels of Scr, BUN, MDA, NF-κB, and increased SOD activity, Sirt1 level, respectively P<0.05; .and the HE staining of the rats in curcumin group showed lower pathological injure as the contrast-induced nephropathy group, which had decreased scores of tubular injure, P<0.05.
Curcumin can obviously alleviate the injure of tubular epithelial cell form iohexol. Protective effects of curcumin to the rats may be through the way of inhibitting oxidative stress and anti-apoptotic to complete. Curcumin could probably protect against acute kidney injury through upregulating expression of Sirt1 in experimental rats.