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The purpose of this study was to investigate whether nobiletin (NOB) exert the therapeutic effect on cardiac fibrosis in high fat diet induced obesity mice.
38 experimental male C57BL mice were randomly assigned into four groups:NC+Vihicle, NC+Nobiletin, WD+Vihicle, WD+Nobiletin. Catheter-based measurements of hemodynamic parameters were performed at 24 weeks after starting nobiletin or vihicle treatment. Nobiletin treatment ameliorated cardiomyocyte hypertrophy and cardiac dysfunction in the high fat diet induced obese mice, as shown by the result of wheat germ agglutinin (WGA) staining and hemodynamic measurements. To investigate whether nobiletin exert the therapeutic effect on cardiac fibrosis, we utilize picro-sirius red staining to detect the cardiac fibrosis in high fat diet induced obese mice, our result indicated that nobiletin treatment could alleviate cardiac fibrosis. Further studies showed that high fat diet induced obese significantly induced the activation of TGF-β/Smad signaling in the cardiac tissues, which was blocked by nobiletin treatment.
Western diet-induced obesity mice could result in lipotoxic cardiomyopathy and consequently cause cardiac remodeling. Nobiletin can reduce body weight of mice and alleviate myocardial fibrosis and cardiac remodeling.
Nobiletin may have an ability to retard the progression of cardiac hypertrophy, and alleviate cardiac fibriosis in high fat diet induced obesity mediated by the blockade of TGF-β/Smad signaling pathways.