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To investigate the relationship between the cardioprotective effect of resveratrol from anoxia/reoxygenation injury and AMPK pathway.
H9c2 cardiomyocytes were divided into normal control group, A/R group and resveratrol pretreatment group. Expression of AMPK was determined by Western blot, and cell viability was measured by MTT method. LDH, SOD and GSH-Px activity were determined by chromometry.
After 12h resveratrol precondition, the expression of AMPK was upregulated in cardiomyocytes (P<0.01). In the group pretreated with resveratrol before A/R, cell viability increased; the activity of LDH in culture medium decreased; the activity of intracellular SOD, GSH-Px increased (P<0.01).
The effect of resveratrol against A/R injury in cardiomyocytes involves AMPK pathway, and at least partly depends on its effect of upregulating the expression of AMPK.