Author + information
- Varsha Wankhade1,2,
- Amar Jadhav1,
- Syed Abrar Ahmad1,
- Chandrikant Chavan2,
- Arundhati Diwan2 and
- Rajesh Badani3
This study focuses on the status of oxidative stress in cardiovascular diseases in Pune population. The main objective was to unravel the antioxidant enzyme status in Pune population among Heart patients.
The present study protocol was approved by Institutional Human Ethics committee. Ten 10 ml blood samples were taken from both control and heart patients with informed consent. Plasma were isolated and stored at -80oC for further use. Proteins were isolated from 100ul plasma samples and stored at -80oC. Catalase enzyme assay was performed e by Aebi (1983) with some modifications. Glutathione peroxidase enzyme assay was performed e by the method of Richard (1976) with some modifications. Glutathione reductase assay was performed by method of David et al. (1983).
Myocardial infraction patients show higher systolic BP (132.33±4.333) than Ischemic heart disease patients (123.0±4.359), and HCM patients (121.867±3.983). Patients of Myocardial infraction heart disease show higher diastolic BP (84.167±5.231) than Ischemic heart disease patients (77.0±4.899) and HCM patients (73.8±2.662) with (p=0.17). Catalase activity (U/mg) was estimated in plasma samples in all participants. Control samples shows higher value (0.286±0.243) than HCM samples (0.205±0.047) with p= 0.27. Patients suffering from IHD show lower levels of catalase (0.149±0.082) than HCM patients and control donors (p=0.89). MI patients show lowest levels of catalase activity than other groups (0.141±0.023) p= 0.3.
The level of GPX in Control samples was 0.017±0.01, whereas in HCM patients the level is 0.007±0.002, MI patients shows lowest levels of GPx (0.004±0.001) than other groups, IHD patients shows higher levels of GPx (0.010±0.003) than other diseased participants. GR in GR levels were higher in control samples (0.011±0.004) than HCM patients (0.009±0.002) p= 0.23, MI patients (0.005±0.001) p= 0.4, and IHD (0.008±0.003) p= 0.77. MI patients show lowest levels of GR than other patient groups.
Patients with myocardial infraction show higher levels of hypertension (both systolic and diastolic).Anti-oxidant enzyme levels were lower in heart patients than control samples indicating higher scores of oxidative stress in heart patients. Positive correlation occurs between heart physiology and catalase levels, as it has found that higher the score of oxidative stress worse is heart physiology.