Author + information
The obesity-induced hypertension is associated with high morbidity and mortality, because it leads to cardiovascular disease. And the prevention and treatment of obesity-induced hypertension is a major obstacle. So, we discuss the effect of chemerin on artery contraction in obesity-induced hypertension using model rats and human aortic smooth muscle cells. Our study will try to identify novel targets, which would provide new ideas for the prevention and treatment of obesity-induced hypertension.
After one week feeding with normal diet, the 8-week-old Wistar rats (n = 40) were randomly divided into two groups. The control group (n=10) was fed with general diet, while the model group (n=30) was fed with high-fat diet for 20 weeks. Body weight, blood pressure and heart rates were measured every 4 weeks. Serum chemerin levels were measured at 0, 4 and 20 weeks. Then, all the rats were sacrificed at the end of the experiments. The effect of chemerin 9 on artery tension in aortic rings was examined by vascular tone detector. The expression of chemerin, CMKLR1, ROCK and P-MYPT1 were detected by Western blot and Immunohistochemistry in arteries and human aortic smooth muscle cells.
After 4 weeks of high-fat diet, The weight, pressure, heart rates and Lee's index of rats were significantly higher than the control group (p<0.05). After 20 weeks, serum LDL-C, epididymis adipose mass/body weight were higher than the control group (p<0.05). However the blood sugar and triglyceride have no significant difference. Chemerin 9 potentiated the artery tension in model group (p＜0.05). The expression of chemerin, CMKLR1, ROCK and P-MYPT1 were much higher in model group, and ROCK 2 were increased by chemerin time-dependently.
The obesity-induced hypertensive rats were success- fully modeled by high fat diet. Chemerin can enhance the vasoconstriction of the hypertensive rats. Chemerin may promote vasoconstriction through ROCK2/P -MYPT1 signaling pathway.