Author + information
- Jia Xu,
- Jinli Liao,
- Yan Xiong,
- Feng Zhu,
- Ziyu Zheng and
- Hong Zhan
Enhanced external counterpulsation (EECP) has a beneficial effect on endothelial progenitor cells (EPCs) which play a pivotal role in endothelial repair following conorary arterial injury. However, the underlying mechanism of effects on endothelial intergrity and EPC signaling pathway involved in it remained to be studied. So, we investigate the effects of EECP on reendothelialization capacity of human circulating EPCs and the related epidermal growth factor homology domain-2 (Tie-2)-dependent signaling pathway in stable angina patients.
86 stable angina patients were enrolled in our study, therein 37 patients received EECP treatment.60 healthy volunteers were selected as control group. The number and activity of circulating EPCs as well as the levels of GM-CSF and NO-VEGF in plasma and cell culture medium were measured in subjects of three group. The protein expression levels of Tie2/PI3K/Akt/eNOS signaling pathway were detected by western blot. In vivo, reendothelialization capacity of human EPCs in different group was detected by transplantation of EPCs in nude mouse model of carotid artery injury.
The number and activity of circulating EPCs was impaired in stable angina patients compared with healthy volunteers and restored in EECP treatment group significantly. In parallel, EECP treatment enhanced levels of NO, VEGF, and GM-CSF of both serum and cultured medium. Transplantation of EPCs treated by EECP facilliated in vivo reendothelialization in nude mouse with carotid artery injury combined with enhanced phosphorylation of Tie2 and Akt protein expression of EPCs.
EECP treatment could enhance the number and reendothelialization capacity of EPCs in stable angina via Tie2/PI3K/Akt/eNOS signaling pathway,at least in part.