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The thymus is an important part of the development and mature of T lymphocytes, and previous studies have shown that T lymphocytes are involved in the progression of hypertension, so this research by detecting the changes of thymus function in hypertension mice, to provide a novelty clinical treatment methods of hypertension.
In this experiment, 8-week-old C57BL/6J mice as research subjects were randomly divided into control group, sham group and hypertension group (the left side of renal artery stenosis model), 20 in each group. At the end of the experiment, the mice were sacrificed and the mice were left to take the mouse thymus. T cell subsets flow cytometry analysis was performed on blood samples, and RNA and DNA extraction from thymus were to detect thymus FoxN1 and T cell receptor rearrangement excision circles expression. HE staining on the left part of thymus tissue.
Our data showed that FoxN1 levels in hypertensive group were lower than those in control group (p<0.05). In the sham operation group, the level of FoxN1 was also down regulated in the early stage (4 weeks), but the expression level was partially restored 8 weeks after operation. The changes of T cell subsets and signal-joint T cell receptor rearrangement excision circles (signal-joint TREC) were similar to the results of FoxN1 expression. The thymus HE staining showed staining showed that the structure of thymic epithelial tissue of hypertensive mice was degenerated, and the boundary of cortex and medulla was not clear and reduction in the number of medullary islets per thymic lobe in the hypertension group.
The study found that the function of thymus was dysfunction in hypertension mice, which might provide a novel target for the treatment of hypertension by improving the function of thymus.
Key words: Hypertension, thymic function, T cell receptor rearrangement excision circles