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Post-infarction induced heart failure (HF) is an end stage with a poor prognosis, but the molecular mechanisms have not been fully elucidated. In the study, we tried to discuss the precise regulatory mechanisms of post-infarction hear failure and to identify key regulatory miRNAs.
We determined the differential miRNA profile in a rat model of post-infarction heart failure through high throughout sequencing and analyzed the data through bioinformatics approaches. We validated the results through qRT-PCR. Expressions patterns of 4 miRNAs in different periods after myocardial infarction were analyzed. Finally, gain- and loss-of-function experiments of rno-miR-184 were conducted in H2O2 treated H9c2 cells.
In the HF sample, 106 miRNAs were differentially expressed compared to the controls. GO and KEGG pathway analysis was conducted to predict the likely roles of these miRNAs. Time-course analysis revealed differential expression patterns of rno-miR-122-5p, rno-miR-199a-5p, rno-miR-184 and rno-miR-208a-3p. Rno-miR-184 was proved to promote apoptosis in vitro.
Differential profile and expression patterns of miRNAs in post-infarction heart failure were found, and the pro-apoptotic role of rno-miR-184 was discovered.