Author + information
- Guo Jianqiang,
- Sujuan Li and
- Jianqiang Guo
To investigate the changing pattern of plasma catestatin concentration during the development and progression of hypertension and its effect of inhibiting the activity of the sympathetic nervous system in hypertensive individuals.
We took Spontaneously Hypertensive Rats (SHR) and their littermates Wistar-Kyoto rats (WKY) as model and these rats were 6, 12, and 16-weeks of age respectively. Heart rate was used as a sign of sympathetic activity. Plasma catesatatin concentration was examined by Enzyme-linked immunosorbent assay. Heart rate was from tail-cuff instrument.
The results showed the plasma catestatin concentration of SHR was higher than that of WKY and with the increase of the age, plasma catestatin concentration was increased gradually in SHR but was not changed in WKY. SHR VS WKY, six-week-old: 1.63±0.65 VS 1.10±0.34ng/ml, P=0.146; twelve-week-old: 1.78±0.32 VS 1.05±0.25ng/ml, P < 0.05; sixteen -week-old: 3.06±0.7 VS 1.18±0.14 ng/ml, P < 0.05; Six-week-old SHR VS sixteen-week-old SHR: 1.63±0.65 VS 3.06±0.7 ng/ml, P < 0.05, twelve-week-old SHR VS sixteen-week-old SHR: 1.78±0.32 VS 3.06±0.7 ng/ml, P < 0.05. Exogenous complement catestatin by the tail vein injection reduced heart rate of SHR, before catestatin injection VS 30min after injection: 415±25 VS 372±14 Beat/min, P < 0.05.
The plasma catestatin level of SHR is significantly higher than that of WKY. With the development and progression of hypertension in SHR, plasma catestatin concentrations are gradually increased. This means catestatin involves in the pathogenesis of hypertension. The fact that catestatin can reduce heart rate of SHR proves that it has a clear inhibitory effect of sympathetic activity in real-hypertensive individuals.