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to study the AAV9 - SERCA 2 a gene transfection on atrial fibrillation animal model of acute heart atrial myocyte effect of L - type calcium channel expression.
60 New Zealand white rabbit as experimental object, USES the pericardium injections transfection AAV9 - SERCA2a gene; The subjects were randomly divided into 3 groups: atrial fibrillation group, the transfection SERCA 2 a gene af group and the control group. Acute atrial fibrillation model after transfection. Using patch clamp and fluorescent immunohistochemical method to detect acute atrial myocyte atrial fibrillation animal model of l-type calcium channel current changes and l-type calcium channel protein expression.
1. Turn SERCA 2 a gene LVDCCa1c protein expression in the atrial myocyte atrial fibrillation group was obviously higher than that of atrial fibrillation group, the difference was statistically significant (P < 0.05), there was no statistically significant difference compared with control group (P > 0.05). 2. Turn SERCA 2 a gene af group of atrial muscle cells on l-type calcium channel current density higher than that of atrial fibrillation group and the control group, the difference was statistically significant (P < 0.05).
turn SERCA 2 a gene therapy can reduce acute atrial fibrillation model calcium overload heart cells, reduce episodes of Ica, L the effects of current density, to prevent electrical remodeling in the heart has the positive significance.