Author + information
Our previous investigation showed that the expression of sarcolipin was elevated in heart of rats with experimental diabetic cardiomyopathy. It was believed that the intracellular calcium homeostasis was related with sarcolipin which regulates SERCA. This study was aimed to investigate the effects of sarcolipin silencing on ventricular arrhythmia in diabetic cardiomyopathy.
Animal model of diabetic cardiomyopathy was established. Adenoviral vector carrying specific siRNA against sln was administrated to animals. The knock-down effect of the siRNA was testified by western blotting and real- time PCR. The ventricular arrhythmias were monitored by ECG.
The adenoviral vector administration significantly down- regulated expression level of sarcolipin in cardiac tissue from animal model of diabetic cardiomyopathy. The incidence of ventricular arrhythmias decreased significantly after sarcolipin silencing.
Silencing of sarcolipin could inhibit ventricular arrhythmias in diabetic cardiomyopathy.