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Atherosclerosis is both a chronic inflammatory disease and a lipid metabolism disorder. Salvianolic acid B is isolated from the root of the Chinese herb Danshen, have been widely used for the prevention and treatment of atherosclerosis. Herein, the purpose of this study was to determine anti-atherosclerotic effects of salvianolic acid B in apolipoprotein E-deficient mices.
Apolipoprotein E-deficient mices were made atherosclerosis by feeding a high cholesterol diet, and simultaneously orally supplemented with high (100 mg/kg) and low (200 mg/kg) doses of salvianolic acid B or vehicle from 0 day to 8 week, Blood samples were collected, weight of mices were examed, serum levels of total Cholesterol(TC), triglyceride(TG), high-density lipoproteincholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), interleukin-6 (IL-6) were measured at 0 day, 1, 4, and 8 week. Morphology and inflammatory marker of atherosclerotic lesions was analyzed by histopathological and biological chemistry methods in mices at 8 week.
Epididymal fat pads and serum IL-6 levels in apolipoprotein E-deficient mices were more reduced by high-dose salvianolic acid B compared with low-dose salvianolic acid B and vehicle treatment mices. Moreover, high-dose salvianolic acid B apolipoprotein E-deficient mices had the lower serum LDL-C levels and atherogenic index (LDL-C/HDL-C) than the other two groups. Fat deposition, intima/media ratio in the aortic areas and percentage of mice with atherosclerotic lesion of high-dose salvianolic acid B apolipoprotein E-deficient mices were decreased more than those of other two groups. The expressions of inflammatory marker NF-κB and macrophage marker F4/80 in the high-dose salvianolic acid B apolipoprotein E-deficient mices atherosclerotic vessels were both diminished more than those of the two groups. There were no differences between the low-dose salvianolic acid B apolipoprotein E-deficient mices and vehicle treatment mices, but they were all different with wild mices.
High-dose salvianolic acid B is associated with reduced levels of several cardiometabolic risk factors by reducing the LDL-C levels and suppressing atherosclerotic inflammation, while a low-dose salvianolic acid B has no these effects. These findings suggest a novel potential mechanistic explanation for atherosclerosis.