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To observe the effect of combined administration of tanshinone IIA and △7-stigmastenol on inflammatory response in Chinese mini swine with coronary atherosclerosis.
48 Chinese mini swines were randomly divided into six groups, with eight in each group. Six groups included the normal control group, the model group, the simvastatin group with does of 1.0 mg/kg, combined administration groups at the same mass ratio with different does of 0.5, 1.0, 1.5mg/kg. Except for the normal control group, all of other groups were fed with high fat diet for 2 weeks. Swines were injured at their left anterior descending artery endothelium by interventional balloons, then were fed with high fat diet for 8 weeks to prepare the coronary atherosclerosis model. In the 8th week, the intravascular ultrasound was used to observe the total coronary artery plaque burden of each group. Inflammatory factors such as hs-CRP, TNF-α, sICAM-1 and IL-6 were detected by ELISA. The expression of NF-κB p65 nuclear translocation was observed by the immunohistochemical method.
Compared with the normal control group, the model group showed significant increase in the total coronary artery plaque burden (P<0.01), and remarkable growth of hs-CRP, TNF-α, sICAM-1 and IL-6 levels (P<0.01). The immunohistochemical staining also showed the significant increase in the NF-κB p65 nuclear translocation of coronary artery of Chinese mini swines in the model group. Compared with the model group, combined administration of tanshinoneIIA and △7-stigmastenol could significantly attenuate atherosclerotic plaque burden (P<0.01), decrease such inflammatory cell factors as hs-CRP, TNF-α, sICAM-1 and IL-6 in serum, and inhibit the NF-κB p65 nuclear translocation of coronary artery (P<0.01). Compared with the simvastatin group, both the median and high dose combined administration group can better lower the level of inflammatory cell factors above (P<0.05 or P<0.01).
Combined administration of tanshinone IIA and △7-stigmastenol can reduce the downstream inflammatory response by controlling NF-κB p65 nuclear translocation, further, inhibit the ocurrence and development of coronary atherosclerotic plaque in Chinese mini swine.