Author + information
- Shan Reai1
To observe the effect of Hypertonic Sodium Chloride Hydroxyethyl Starch 40 (HSH) on the expression of neuron specific enolase (Neuron- specific enolase, NSE)after nerve cells injury and explore the neural protective mechanism of HSH in acute cerebral ischemia reperfusion (I/R) rats.
20 male SD rats were randomly divided into four groups: shamed-operation group (group A), model group (group B), HSH 4ml/kg group (group C) and HSH 8ml/kg group (group D). Rat focal cerebral I/R injury model was simulated by middle cerebral artery occlusion (MCAO). Fifty minutes after modeling, group C, D were treated with HSH and group A, B were treated with physiological saline by sublingual vein injection. 24 hours after reperfusion, nerve function was evaluated by using Longa's score-line, Expression alteration of NSE in each group rats' same injured side brain tissues were detected by Western Blotting.
Compared with group A, neurological behavioral score was increased in group B(1.6±0.7 vs 0±0, p<0.01); Compared with group B, neurological behavioral score were both reduced in group C (0.6±0.5 vs 1.6±0.7, p<0.01) and D (1.0±0.4 vs 1.6±0.7,p<0.01). Compared with group A, the expression of NSE of group B (0.78±0.01 vs 0.17±0.01) increased, the difference was significant (P < 0.01). Compared with group B, the expression of NSE of group C (0.51±0.02 vs 0.78±0.01) and group D (0.59±0.01 vs 0.78±0.01) decreased, the difference was significant (P < 0.01).
HSH could improve neurological behavioral score and Western blotting analysis showed that HSH could decrease the NSE expression, which shows that HSH has a protective effect on rat cerebral I/R. It suggests that the protective effect of HSH may be related to the decrease of NSE expression.