Author + information
- Shan Reai1
To explore the effect of Hypertonic Sodium Chloride Hydroxyethyl Starch 40 (HSH) on the expression of heat shock protein 70(Heat Shock Protein 70, HSP70)after nerve cells injury and discuss the neural protective mechanism of HSH in acute cerebral ischemia reperfusion (I/R) rats.
20 male SD rats were randomly divided into four groups: shamed-operation group (group A), model group (group B), HSH 4ml/kg group (group C) and HSH 8ml/kg group (group D). Rat focal cerebral I/R injury model was simulated by middle cerebral artery occlusion (MCAO). Fifty minutes after modeling, group C, D were treated with HSH and group A, B were treated with physiological saline by sublingual vein injection. 24 hours after reperfusion, nerve function was evaluated by using Longa's score-line, Expression alteration of HSP70 in each group rats' same injured side brain tissues were detected by Western Blotting.
Compared with group A, neurological behavioral score was increased in group B(1.6±0.7 vs 0±0, p<0.01); Compared with group B, neurological behavioral score were both reduced in group C (0.6±0.5 vs 1.6±0.7, p<0.01) and D (1.0±0.4 vs 1.6±0.7, p<0.01). Compared with group A, the expression of heat shock protein 70 was increased in groupB(0.66±0.10 vs 0.42±0.13, p<0.01); Compared with group B, the expressions of heat shock protein 70 were both increased in group C (0.81±0.05 vs 0.66±0.10, p<0.01) and D(0.76±0.04 vs 0.66±0.10, p<0.01), the difference of group C was significant (p<0.05).
HSH could improve neurological behavioral score and Western blotting analysis showed that HSH could increase the HSP70 expression, which shows that HSH has a protective effect on rat cerebral I/R. It suggests that the protective effect of HSH may be related to the increase of HSP70 expression.