Author + information
- Maoxiao Nie,
- Yunfeng Yan,
- Xinhe Li,
- Tingting Feng,
- Xin Zhao,
- Mingduo Zhang and
- Quanming Zhao
We investigated the effect of different concentrations of elastase on the aneurysm formation rate in the abdominal aortic aneurysm (AAA) model induced by low-pressurized abdominal aortic perfusion of elastase in rabbits.
Fifty male New Zealand white rabbits were randomly divided into five groups (group A, B, C, D and E), with 10 rabbits in each group. Using a double-lumen catheter, 10 μl of normal saline was perfused into the abdominal aorta in group A. For the other four groups, 1 U/ml, 10 U/ml, 100 U/ml or 200 U/ml elastase was perfused using a double-lumen catheter. The animals were perfused for 7 min, and then the abdominal aorta was flushed three times with normal saline. Doppler ultrasound examinations of the abdominal aorta were performed before surgery and on day 14 after surgery. The rabbits were sacrificed on day 14 after surgery, and the perfused segment of the abdominal aorta was observed visually and after staining.
Nine rabbits in group A survived without obvious dilation of the perfused segment, and the aneurysm formation rate was 0%. Nine rabbits survived in group B; the perfusion segment dilation rate was 21.4-29.7%, and the aneurysm formation rate was 0%. Nine rabbits survived in group C, and the perfusion segment dilation rate was 31.2-55.3%; the dilation rate was greater than 50% in 3 rabbits, which satisfied the criterion of aneurysm formation in the abdominal aorta. Therefore, the aneurysm formation rate of group C was 33.3%. Nine rabbits survived in group D; the perfusion segment dilation rate was 102.5-146.8%, and theaneurysm formation rate was 100%. In group E, 4 rabbits survived, and the perfusion segment dilation rate was 241.5-255.2%, with an aneurysm formation rate of 100%. However, the survival rate was only 40% on day 14 after surgery.
Abdominal aortic perfusion of 100 U/ml of elastase is an easy and feasible method to induce AAAs in rabbits with a high aneurysm formation rate, low death rate, and short perfusion time.
This study was supported by the National Natural Science Foundation of China; No. 81355392