Author + information
- Lizhen Hao,
- Xingzhou Gao,
- Hong Chang,
- Houwang Jiang and
- Xinlou Chai
To study the effect of Huoluoxiaoling Dan on the apoptosis of human umbilical vein endothelial cells in vitro induced by hydrogen peroxide (H2O2), and to explore the mechanism.
Human umbilical vein endothelial cells were cultured in vitro, Huoluoxiaoling Dan water solution pretreated the cells for 24h, and then used the H2O2 on HUVECs for 4h. MTT was used to detect the growth activity of the cells, and flow cytometry was used to detect the apoptosis rate, the protein GRP78, XBP1, caspase-3 and caspase-9 were detected by Western blot.
Hydrogen peroxide could decrease the activity of endothelial cells in a dose-dependent manner, and induce apoptosis. Among them, after 500 μmol/L H2O2, cell proliferation was obviously inhibited, and the content of caspase-3, caspase-9 and GRP78, XBP1were significantly increased(P<0.01).After the addition of Huoluoxiaoling Dan, the survival rate of cells increased, and compared with the H2O2 model, 80ug/ml of Huoluoxiaoling Dan pretreatment could significantly inhibit apoptosis, and decreased the expression of caspase-3, caspase-9 and GRP78, XBP1 (P<0.01).
Huoluoxiaoling Dan can inhibit the apoptosis of endothelial cells induced by hydrogen peroxide, which may be related to the down-regulation of the endoplasmic reticulum stress related proteins.