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To observe the effect of Hypertonic Sodium Chloride Hydroxyethyl Starch 40 (HSH) on neurological deficits scores, degree of encephaledema, cerebral infarction volume andexpression of MMP-9 and Laminin in a rat model of ischemia/reperfusion(I/R) injury.
48 male SD rats were randomly divided into four groups: shamed-operation group (group A), model group (group B), HSH 4ml/kg group (group C) and HSH 8ml/kg group (group D). Rat focal cerebral I/R injury model was simulated by middle cerebral artery occlusion (MCAO). 120 minutes after modeling, group A, B were treated with physiological saline and group C, D were treated with HSH by sublingual vein injection. 24 hours after reperfusion, nerve function was evaluated by using Longa's score-line in every rat, six of every group'sdegree of encephaledema would be observed and TTC staining would be applied to measure the cerebral infarction volume after taking out the brain. Expression of MMP-9 and Laminin were detected with western blotting in ischemic penumbra of the other six after observing the degree of encephaledema.
The percentage of cerebral infarction volume and neurological behavioral score of group B was more than these of group A(p<0.001)with an obviousencephaledema. The expression of MMP-9 of group B significantly increased versus group A (p<0.05),but Laminin decreased (p<0.05). Compared with group B,The percentage of cerebral infarction volume and neurological behavioral score of group C, D dereased (p<0.001)with a catabatic degree of encephaledema. The expression of MMP-9 of group C significantly decreased versus group B(p<0.05), but Laminin increased (p<0.05). Group D had no obvious change.
MMP-9 protein would be more activated and accelerate the degradation of Laminin protein so that BBB's permeability increased on rat cerebral I/R. Meanwhile, encephaledema and cerebral infarction happened and neurological behavioral score increased. HSHcan significantly reduce BBB's permeability, cerebral infarct volume, degree of encephaledema and neurological dysfunction in rat I/R injury models. These findings indicated that MMP-9 and Laminin may be involved in the protective mechanisms of HSHagainst cerebral ischemia/reperfusion injury. According to the dose of HSH, 4 ml/kg is better than 8 ml/kg.