Author + information
- Ailifeire Maimaiti,
- Fen Liu,
- Yitong Ma,
- You Chen and
- Bangdang Chen
MicroRNAs (miRNAs), approximately 22-nt-long noncoding RNAs, suppress gene expression by binding to the 3' untranslated regions (UTRs) of target mRNAs. Data reveals that miRNAs could be serologically detected which probably could be used as potential biomarkers of cardiovascular diseases. In this study, we performed miRNA arrays analysis on Uyghur and Han patients suffering from acute myocardial infarction (AMI) and stable angina pectoris to obtain differential miRNAs expression profile between two ethnic groups under stable(stable angina pectoris) and unstable (AMI) status of coronary artery disease(CAD).
20 CAD patients in total were selected after angiographic validation of CAD, including 5 Han AMI (HA) patients, 5 Uyghur AMI (UA) patients, 5 Han stable angina pectoris (HS) patients and 5 Uyghur stable angina pectoris (US) patients. First of all, we isolated RNA from patients' EDTA-plasma by using mirVana miRNA Isolation Kit (ABI), then the RNA products were pre-amplified with Megaplex? RT Primers Human Pool A v2.1(ABI) to elevate the RNA concentration. Subsequently, RNA products were reverse transcribed by using the TaqMan microRNA Reverse Transcription (RT) kit (ABI) according to the protocol of the manufacturer. At last, the miRNA expression was detected with TaqMan Human MicroRNA Array by quantitative PCR for the certain miRNAs. Comparisons were made between HA and HS groups as well as UA and US subjects. Significantly differed (RQ>2.0 or RQ≤ 0.5, p<0.05) miRNAs were assessed for correlation with different status of CAD.
Two up-regulated miRNAs (hsa-miR-342-5p and hsa-miR-362-3p) and seven down-regulated miRNAs (hsa-let-7e, hsa-miR-15b, hsa-miR-224, hsa-miR-26a, hsa-miR-301a, hsa-miR-367 and hsa-miR-99b) were identified in HA patients compared to HS patients (miR-342-5p, RQ=2.4501; miR-362-3p, RQ=3.0752; let-7e, RQ=0.0875; miR-15b, RQ=0.2861; miR-224, RQ=0.2221; miR-26a, RQ=0.2314; miR-301a, RQ=0.2891; miR-367, RQ=0.2778; miR-99b, RQ=0.2399; p<0.05 for all miRNAs). Among Uyghur subjects, we detected one up-regulated miRNA (hsa-miR-450a) and three down-regulated miRNAs (hsa-miR-140-3p, hsa-miR-196b, hsa-miR-367) comparing UA to US subjects (miR-450a, RQ=2.0031; miR-140-3p, RQ=0.3755; miR-196b, RQ=0.3672; miR-367, RQ=0.2628; p<0.05 for all). Taken together, miR-367 was down-regulated in both ethnic AMI groups compared to stale angina pectoris groups.
There are several differentially expressed miRNAs in different phases of the same disease in different ethnic groups, which may imply different pathology processes due to the heterogeneity of genetic susceptibility and miRNA regulatory system. Nevertheless, miR-367 was found to be differentially expressed in both two ethnic groups, which probably could be explained by the hypothesis that miRNAs are more related to certain states human body subjected to than human races. The significance of these findings needs to be further elucidated.