Author + information
- Liao Jinli,
- Jia Xu,
- Yan Xiong,
- Ren Jiang,
- Ziyu Zheng and
- Hong Zhan
To investigate the contribute of antibody of recombinant cyclophilin A of mouse on reducing neutrophil extracellular traps formation, therefore improving inflammatory injury of myocardium and cardiac function.
Healthy New Zealand rabbit was immunized with 1 mg rMoCyPA protein three times at an interval of two weeks to obtain anti-rMoCyPA serum.Specific IgG values was measured to determine immune response provoked by rMoCyPA. Male Balb/c mice were randomed into 3 groups:Control group,LPS group,LPS+anti-rMoCyPA group.The rMoCyPA-immunized serum was complement-inactivated and injected via caudal vein in recipient rats 2h after LPS administration.72h mortality and cardiac microscopic changes were evaluated.HE staining were used to assess inflammatory damage at different time points.The mRNA expression level of functional tissue factor, IL-8, IL-6 and TNF-α of myocardium were detected by RT-PCR.The NETs were observed by laser confocal microsope.The ventricular function was evaluated in mice by echocardiography.
Anti-rMoCyPA serum significantly reduced the 72h mortality of septic mice, alleviated the macroscopic and microscopic damages of inflammatory reaction.The functional tissue factor,IL-8, IL-6 and TNF-α mRNA expression level of myocardium were reduced in mice treated by anti-rMoCyPA serum compared with LPS group and control group.Less NETs were observed with anti-rMoCyPA intervention combined with improved state of myocardial dysfunction,characterized by increased LV ejection fraction.
Through down-regulating NETs formation, anti-rMoCyPA serum could alleviated inflammatory injury of myocardial tissues and restore LV function in LPS-induced septic mice.