Author + information
To observe the effect of salidroside (Sal) on the myocardial ischemia and the apoptosis pathway of both death receptors and mitochondria in acute exhausted rats.
Male SD rats were randomly divided into 4 groups (n=6): control group (Con), exhaustive exercise group (EE), low-dose and high-dose Sal pretreatment exhaustived swimming group (SEL, SEH). Groups were given Sal solution (15 or 30mg/kg/d) or the amount of normal saline through intraperitoneal injection 15 days. Con, EE group 0.9%NaCl were given (3ml/kg/d) intraperitoneal injection for 15 days. After the Sal pretreatment, the Con group did not do any exercise. EE, SEL and SEH groups' rats were forced to swim until they were exhausted. The changes of ischemia modified albumin (IMA) in serum were observed by ELISA.TUNEL and semi quantitative analysis method was used to detect the myocardial cell apoptosis index (AI). Immunohistochemical method was used to detect myocardial Bax/Bcl-2. Western blot method was used to detect the expression of myocardial Fas Cyto-c, Caspase-3, Caspase- 8, and Caspase- 9.
(1) The apoptosis index (AI) in EE group was increased significantly (EE: 36.00±2.00 VS Con: 27.00±4.32, P<0.05) compared with the Con group. Although the SEL group (35.60±8.26) was lower than the EE group, it was no statistical different (P>0.05). The SEH group (23.83±3.812) was the lowest than the EE and SEL group, the difference was statistically significant (P<0.05). (2) In terms of the serum levels of IMA, the EE, SEL and SEH group were significantly increased than Con group (P<0.05), the SEH and SEL group were reduced than the EE group (P<0.05), while the SEH group was significantly lower than that the SEL group (P<0.05). (3) Compared with the Con group in the Bax immunohistochemistry score, the EE and SEL group were increased (P<0.05). The SEH group was reduced evidently than the EE and SEL groups, and not significant different from the Con group. (4) Compared with the Con group, EE group, SEL group in the Bcl-2 immunohistochemistry score was significantly lower than the Bcl-2 group (P<0.05). The SEH group was increased significantly than the EE and SEL groups, and was slightly higher than the Con group but no statistical difference. (5)The Fas, Cyto-C and Caspase-3, Caspase-8, Caspase-9 myocardial expression quantity of the EE group, the SEL group and the SEH group wre increased significantly than the Con group (P<0.05). The EE group, the SEL group and the SEH group were reduced, and the difference between each other was statistically significant (P<0.05).
Salidroside can significantly reduce the myocardial ischemia and reduce myocardial cell apoptosis in exhaustive exercise rats. The mechanism may be related to inhibit the expression of Bax, Fas, Cyto-C, Caspase-3, Caspase-8, Caspase-9 and increase Bcl-2.