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This study was designed to investigate the cardioprotective effects and potential mechanism of SM and DO on rat myocardial ischemia/reperfusion (MI/R) injury.
Sprague-Dawley rats were pretreated with Radix salviae miltiorrhizae (SM), aqueous extract of Lignum dalbergiae odoriferae (DOA) and volatile oil of Lignum dalbergiae odoriferae (DOO), either as monotherapy or in combination for 7 days, then subjected to 30 min of ischemia followed by 180 min of reperfusion. Infarct area, myocardial marker enzymes, apoptosis, oxidative stress and inflammatory factors were determined by proper methods. Moreover, metabonomics based on GC-TOF-MS was used to identify the therapeutic effect of these TCM.
he results showed that SM, DOA and DOO ameliorated cardiac function respectively, and this effect was further strengthened by their combinations. Among all combinations, SM+DOO showed predominant potential (P<0.01) to improve electrocardiogram and heart rate, reduce heart weight index and myocardial infarct size, decrease the levels of CK-MB and LDH. Moreover, metabonomics-based findings such as the PCA and PLS-DA score plot of metabolic state in rat serum correlated well to the preceding results, confirming that SM+DOO exerted synergistic therapeutic efficacies to exhibit better effect on MI/R injury rats compared with other pretreatment groups. Furthermore, the maximum effective point of SM+DOO was 30min and the minimum effective point was 180min. In addition, SM+DOO group markedly (P<0.01) reduced the number of TUNEL-positive cells, the levels of TNF-α, IL-6 and MDA, whileincreased the activity of SOD both in serum and tissue.
These results indicated that SM+DOO have combined effects that are highly effective than single pretreatment against MI/R injury rats. This effect might be achieved partly through anti-apoptosis, anti-oxidant and anti-inflammatory. Thus SM+DOO might be an effective and promising medicine for both prophylaxis and treatment of ischemic heart disease.