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To investigate relaxant effect of salidroside (SAL) on rat mesenteric artery, and to explore its calcium regulation mechanisms．
Tension was measured by DMT 620M system to evaluate the relaxant effect of SAL on rat mesenteric artery rings. Calcium deprivation and calcium addition were used . Besides, for protein assay, 20 adult male Sprague Dawley SD rats were randomly divided into control group (Con), salidroside group (SAL).The protein expression of IP3R1 and RyR2 were determined by Western blotting.
①With calcium deprivation and calcium addition, SAL relaxed endothelium-denuded mesenteric artery precontracted by PE (1μmol·L-1) (P<0.01 in 10-5 mol·L-1 SAL), while SAL had no effect on the increasing tension of mesenteric artery induced by adding CaCl2.② In SAL group SAL significantly reduced the expression of IP3R1, and increased the expression of RyR2 (P<0.01).
These results indicate that SAL mainly relaxes mesenteric artery by inhibiting the expression of IP3R1 and then inhibition of intracellular calcium release, in spite of the increase of theexpression of RyR2. Besides, this vasodilation effect of SAL may be not associated with influx of extracellular calcium.