Author + information
- Farzad Shidfar1,
- Samira Amani1,
- Mohammadreza Vafa1,
- Ramin Shekarriz2,
- Sharieh Hosseini3,
- Shahrzad Shidfar4,
- Mohammadreza Eshraghian5 and
- Neda Mousavi1
- 1school of health, Iran University of Medical Sciences, Tehran, Iran
- 2School of Medicine, Mazandaran University of medical sciences, Sari, Iran
- 3Faculty of Phrmaceutical chemistry, Pharmaceutical sciences Branch, Islamic Azad University, Tehran, Iran
- 4Memorial Hospital, University of Massachusetts, Worcester, U.S.A
- 5School of Health, Tehran University of Medical Sciences, Tehran, Iran
Numerous studies have demonstrated that tissue deposition of iron following prolonged high dose of oral supplementation for treatment of iron deficiency anemia (IDA) leads to body iron overload and oxidative stress, which starts the process of atherosclerosis. This study aimed to determine the effect of iron supplementation in combination with docosahexaenoic acid (DHA) on the cardiovascular disease risk based on paraoxonase-1 (PON-1)(by spectrophotometry), high-sensitivity C-reactive protein (hs-CRP) (by Immunofluorescence), and ApoB/ApoA-I ratio (by Turbidimetry) in women with IDA.
In a double blind randomized controlled trial, 76 women with IDA, aged 15-45 years, were included. The patients were randomly assigned to receive 500 mg of DHA supplement or placebo with an iron tablet (a ferrous sulfate tablet contained 50 mg elemental iron), once daily for 12 weeks. Participants were instructed to maintain an isocaloric diet, continue their previous eating habits, and not to change their routine physical activities during the study period. The participants were assessed by measurement of the serum iron (by Spectrophotometer), ferritin (by chemiluminescent immunoassay), PON-1, hs-CRP levels, and the ApoB/ApoA-I ratio at the beginning and end of study.
Serum hs-CRP decreased in the DHA-supplemented group (p?=?0.036), and ApoA-I decreased in the placebo group (p?=?0.013). No significant difference was detected for the serum PON-1 concentration and the ApoB/ApoA-I ratio in two groups.
Iron supplementation combined with DHA may have favorable effects on serum hs-CRP in women with IDA.