Author + information
- Rui-Xing Yin,
- Xia Chen and
- Qing-Hui Zhang
The solute carrier family 39 (zinc transporter) member 8 gene (SLC39A8) on chromosome 4q24 is one of the novel identified loci. The association of the SLC39A8 rs971752 single nucleotide polymorphism (SNP) and serum lipid profiles has not been studied previously in the Chinese populations. The present study aimed to detect the association of the SLC39A8 rs971752 SNP and several environmental factors with serum lipid levels in the Jing and Han populations.
This study included 741 unrelated subjects of Jing nationality and 742 unrelated participants of Han nationality who live in the same regions. They were randomly selected from our previous stratified randomized samples. Genotyping of the SLC39A8 rs971752 SNP was performed by polymerase chain reaction and restriction fragment length polymorphism, and then confirmed by direct sequencing.
The genotypic distribution in both Jing and Han was conformed with the Hardy-Weinberg equilibrium. The frequency of SLC39A8 rs971752G allele was higher in Han than in Jing (12.7% vs. 9.6%, P < 0.01). The frequencies of AA, AG and GG genotypes were 82.2%, 16.5% and 1.3% in Jing, and 75.6%, 23.3% and 1.1% in Han (P < 0.01); respectively. Serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels in Jing were different between the AA and AG/GG genotypes (P < 0.05-0.01). The G allele carriers had higher TG levels, and lower serum TC, HDL-C and LDL-C levels than the G allele non-carriers. Subgroup analyses showed that serum levels of TC, HDL-C and LDL-C in Jing males and TG levels in Jing females were different between the AA and AG/GG genotypes (P < 0.05 for all), the G allele carriers had higher serum TG levels in Jing females, and lower serum TC, HDL-C and LDL-C levels in Jing males than the G allele non-carriers. Multiple linear regression analysis showed that serum TC levels in the combined population of Han and Jing; and TC, HDL-C, LDL-C levels in Jing but not in Han were correlated with the genotypes of the SLC39A8 rs971752 SNP (P < 0.05). When the correlation of serum lipid parameters and the genotypes was performed according to sex subgroups, we revealed that serum TC, HDL-C and LDL-C levels in Jing males but not in females were correlated with the genotypes (P < 0.05). Serum lipid parameters were also correlated with several environmental factors such as age, gender, height, weight, BMI, waist circumference, alcohol consumption, cigarette smoking, blood pressure and blood glucose in both ethnic groups (P < 0.05-0.001).
The association of the SLC39A8 rs971752 SNP and serum lipid levels was found in the Jing but not in the Han populations. The G allele carriers had higher serum TG levels in Jing females and lower TC, HDL-C and LDL-C levels in Jing males than the G allele non-carriers. These findings suggest that the association between the SLC39A8 rs971752 SNP and serum lipid levels might have ethnic- and/or sex-specificity.