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The aim of this study is to investigate the correlation between the BDNF/TrkB signaling and the patients with myocardial infarction among adultescent.
According to the results of coronary artery angiography,225 participants were divided into four groups: control group(Normal coronary angiography, n=58); angina pectoris group I (AP I,No greater than 50% of lumen stenosis, n=45); angina pectoris group II (AP II,greater than 50% of lumen stenosis, n=42); miocardial infarction group(MI, including acute myocardial infarction, AMI, n=38 and old myocardial infarction, OMI, n=42). Clinical and biochemical characteristics are collected at admission. Participants were advised to refrain from eating and drinking for at least 12 h before collecting vein blood specimen. The level of serum BDNF, TrKB, VE-cad, ICAM-1, eNOs, iNOs, VEGF-A were measured by enzyme-linked immunosorbent assay(ELISA). The total RNA were isolated with TRIzol (Invitrogen), the detection of BDNF and TrKB gene expression was performed by reverse transcription-polymerase chain reaction (RT-PCR).
At baseline,there were no significant differences of these basic indictors in the four groups except for cholesterol and triglyceride. In the AP group and MI group, the level of serum BDNF, TrKB, eNOs were decreased compare to the control group, furthemore, it decreased significantly in AMI group (P < 0.05); however, the level of serum iNOs, ICAM-1, VE-cad, VEGF-A were increased, particularly, there had a significant difference between the MI group and control group (P < 0.05). Moreover, BDNF and TrKB had a positive correlation with eNOs (P < 0.05) and a negative correlation with ICAM-1 and VE-cad (P < 0.05). Meanwhile, the level of serum BDNF also had a significant difference between the AMI group and OMI group (P < 0.05).
We demonstrate that the decrease of BDNF and TrKB involved in the formation and development of myocardial infarction among adultescent and BDNF is a protective factor for myocardial infarction.