Author + information
- Sayed Ali Sheikh1,
- Ke Xia1,
- Fei Li1,
- Peng Liming1,
- Xiaoxiao Mao1,2,
- Umme Salma3,
- Jun Peng4 and
- TianLun Yang1
- 1Department of Cardiology, Xiangya Hospital, Central South University, Changsha, Hunan, China
- 2Cardiovascular Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
- 3Department of Gynecology and obstetrics, Xiangya 3rd Hospital, Central South University, Changsha, Hunan, China
- 4Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, China
The aims of this study were to evaluate the diagnostic and prognostic value of circulating miRNAs (miR-208b, miR-499 and miR-378) in acute NSTEMI and STEMI patients.
40 acute NSTEMI patients, 40 acute STEMI patients, 80 healthy subjects and 16 twelve week-old C57BL/6 mice AMI were enrolled in this study. AMI patients were diagnosed according to ESC/ACCF/AHA/WHF guidelines. Total RNA was isolated from human and mice plasma by using TRIZOL reagent. Real-time quantitative reverse-transcription PCR analysis was carried out to examine the expression of miRNAs in plasma. To investigate the diagnostic and prognostic accuracy of circulatory miRNAs for AMI, receiver operating characteristics (ROC) curve analysis was performed.
We found that plasma miR-208b and miR-499 levels were significantly up-regulated, while expression of plasma miR-378 level was markedly down-regulated in acute NSTEMI and acute STEMI patients compared with healthy subjects (p < 0.001). Plasma miR-208b and miR-499 levels were highest expression observed at 24h after primary PCI, then gradually decreased towards its baseline at 5days and 10days after primary PCI compared with NSTEMI and STEMI patients (p < 0.001). In contrast, plasma miR-378 level was lowest expression observed at 24h after primary PCI, afterward gradually increased towards its normal level at 5days and 10 days after primary PCI as compared with NSTEMI and STEMI patients (p < 0.001). We also found that plasma miR-208b and miR-499 levels were significantly elevated, whereas, miR-378 level was obviously decreased in Mice at 6h and at 12h of AMI compared with controls (p < 0.001). Receiver operating characteristic curve analysis revealed that the respective areas under the curve (AUC) for circulating miR-208b, miR-499 and miR-378 were 0.956, 0.948 and 0.979 in NSTEMI patients and 0.982, 0.973 and 0.971 in STEMI patients compared with healthy subjects.
Our findings suggested that up-regulated circulating miR-208b and miR-499, and down-regulated miR-378, may be used as a novel and sensitive diagnostic or prognostic biomarker for AMI patients.