Author + information
- Jiang Minghong and
To explore the CyclinA in the rat vascular smooth cells and the expression of origin recognition complex-1 (ORC1).
VSMC of thoracic aorta in rats was obtained by the adherence method of tissue culture. The cell synchrony was obtained by the method of double-thymidine block. In different cell cycles of VSMC, the expression of ORC1 mRNA was determined by RT-PCR and the protein expression of ORC1 was observed by flow cytometry.
Synchronized VSMCs were obtained by the method of double-thymidine block,colchicine treatment and serum starvation . Synchronized growth was monitored by flow cytometry. All the synchronized VSMC's distribution ration were 89.22％ (±3.54%) at G0 phase, (66.74±7.16％) at G1/S phase, (63.24±4.06)％ at S phase and (51.64±11.18)％ at G2/M phase. The expression of ORC1 mRNA and CyclinA in a quiescent stage of VSMC was not significantly found, After synchronized, peaked(50.4%) at G2/M of CyclinA, and decreased(1.03%) at G2/M of ORC1, CyclinA in preventing the ORC1 from bind during G2/Mphase. The expression of ORC1 protein and CyclinA had a same change as ORC1 mRNA and CyclinA in different cell cycles of VSMC by flow cytometry (P<0.001).
CyclinA may be an important regulative factor at the initiation of ORC1 in VSMC.