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To evaluate gene expression differences of the complement system in peripheral blood mononuclear cells from patients with acute myocardial infarction (AMI), stable angina pectoris (SA) and the controls.
20 patients with AMI, 20 patients with SA and 20 healthy volunteers were recruited into the study.
Expression of twenty-one genes encoding complement components was detected. In AMI patients, expression of genes encoding C1qα, C1qβ, C1qγ, C1r, Factor P and C5 was significantly higher than SA patients and controls respectively (p < 0.05), whereas C1s mRNA in AMI patients was lower than controls (p < 0.05). In AMI patients, the complement receptors, including CR1, integrin αM, integrin αX, integrin β2, C5aR and CRIg, and the regulators, CD46, CD55 and CD59 were all significantly up-regulated compared with both SA and control group(p < 0.05),while expression of receptor CR2 in AMI and SA patients was significantly down-regulated compared with the controls (p < 0.01). There was no statistical difference of gene expression in complement components and regulators between SA patients and controls.
In AMI patients, the mRNA expression of complement components, receptors and regulators were unbalanced, suggesting the dysfunction of the complement system, which reduces the ability of MAC-induced cell lysis in AMI patients finally, while the complement system in SA patients is inactivated.