Author + information
- Andres Valencia1,
- Athanasios Peppas2,
- Yanping Cheng1,
- Jenn McGregor1,
- Gerard Conditt3,
- Greg Kaluza1 and
- Juan Granada1
There is currently no animal model adequately mimicking complex calcific peripheral atherosclerotic disease for the purpose of testing novel endovascular therapies.
We developed a porcine model of calcific peripheral lesion by implanting custom-designed collagen-based scaffolds that features an even distribution of calcium longitudinally and circumferentially. The 2cm long scaffolds were implanted in bilateral superficial femoral arteries of 4 domestic swine and survived for 21 days. Quantitative vascular angiography (QVA) was performed at implant and termination, Doppler ultrasound was performed weekly, and histological analysis was conducted on harvested arteries containing the inserts.
Weekly Doppler ultrasound confirmed patency in all implants with an average Peak Systolic Velocity of 177±16cm/s at 21 days. QVA %Diameter Stenosis was 42 ±9% post procedure and 59±18% at 21 days with no total occlusions. Histological analysis showed well integrated implants evidenced by advanced endothelialization (3.88 ± 0.25 on a 0-4 score) with widespread calcification (mean 3.55±0.32 on a 0-4 score) within the collagenous matrix (Figure).
Implantation of a hollow collagen+calcium insert in porcine femoral arteries produced a thick-walled calcified vascular conduit that was stenotic while retaining patency. Overall, the vessels implanted with the hollowed insert consistently produced a stenotic calcified lesion that is considered to be a viable and reproducible lesion model, promising for evaluation of drug coated balloons, atherectomy and other peripheral technologies.
ENDOVASCULAR: Peripheral Vascular Disease and Intervention