Author + information
- Wanpitak Pongkana,b,
- Liang Xua,b,
- Osamu Takatoria,b,
- Naoto Nagataa,b,
- Yinhua Nia,b,
- Mai Nakayamaa,b,
- Siriporn Chattipakorna,b,
- Soichiro Usuia,b,
- Tsuguhito Otaa,b and
- Nipon Chattipakorna,b
Background: β-cryptoxanthin (BX) and astaxanthin (AX) are the antioxidant carotenoid pigments which inhibit lipid peroxidation as potently as vitamin E (Vit-E). Although high levels of these carotenoids and vitamin E have been linked to low risk of cardiovascular disease, their roles in cardiac ischemia-reperfusion (I/R) injury are unclear. We hypothesized that acute BX and AX treatments reduce the infarct size caused by I/R injury via reducing oxidative stress and cardiac mitochondrial dysfunction.
Methods: Male C57BL/6 mice (n=36) were randomized to receive vehicle, BX, AX or Vit-E at dosage 50 mg/kg each by gavage feeding 2 hours prior to I/R injury. Cardiac I/R was performed by 30-min LAD ligation, followed by 120-min reperfusion. The infarct size, mitochondrial function, apoptotic process and oxidative stress were determined.
Results: All treatments reduced the infarct size, Bax/Bcl-2 ratio, TBARS and attenuated mitochondrial dysfunction, compared to control groups (Figure). Although AX and Vit-E shared similar efficacy in cardioprotection, BX exerted higher efficacy by reducing the infarct size by 60%. This benefit was due to its greater efficacy in markedly reducing mitochondrial depolarization, mitochondrial swelling, Bax/Bcl-2 ratio and plasma TBARS (Figure).
Conclusions: β-cryptoxanthin exerts higher cardioprotective efficacy against I/R injury than astaxanthin and vitamin-E via reducing the infarct size, apoptosis, oxidative stress and cardiac mitochondrial dysfunction.
Poster Hall, Hall C
Friday, March 17, 2017, 3:45 p.m.-4:30 p.m.
Session Title: Revascularization and Myocardial Preservation in Acute and Stable Ischemic Heart Disease
Abstract Category: 3. Acute and Stable Ischemic Heart Disease: Therapy
Presentation Number: 1165-312
- 2017 American College of Cardiology Foundation